Figure 5
From: Target site specificity and in vivo complexity of the mammalian arginylome
Mouse ATE1-1 exhibits complex specificity toward randomly selected peptide targets. (A) Two-sample logo of the arginylated peptides in the literature set against the (restricted) unlabeled peptides. Two Sample Logo was run with default parameters and color settings (no Bonferroni correction was applied). (B) Cropped image of the autoradiogram showing R incorporation as the 14C signal (gray or black after the film exposure), using peptides with potential side chain D/E target sites (top row), N-terminal D/E target sites with different context in the adjoining residues (middle row), and randomly designed peptides with variable target sites immobilize on the same membrane (bottom row) performed in two independent repeats (see Fig. S3 for the original uncropped image). The peptide sequences are shown underneath. The last three positions in the array were left empty for the negative control.
