Figure 6
From: Target site specificity and in vivo complexity of the mammalian arginylome
Arginylation of N-terminal D or E occurs independently of the specific order of amnino acid residues around the target site. Cropped image of the autoradiogram showing R incorporation as the 14C signal (gray or black after the film exposure), using pairs of peptides with the same amino acid sequence, synthesized in the original (top row) and shuffled (bottom row) order of the residues. In all cases except two, shuffling did not affect the arginylation signal on the peptide. Two repeats are shown for each reaction.
