Figure 5

Effects of tHGA on the phosphorylation of AKT in growth factor-induced hBSMCs. hBSMCs were serum-starved overnight before co-treatment with growth factors and tHGA or triciribine (TCN) for 1 hour. Phosphorylation of (a) AKT (Ser473) and (b) AKT (Thr308) in growth factor-induced hBSMCs upon tHGA treatment were assessed through immunoblotting. Proteins were immunoblotted with respective antibodies and the protein bands were quantified by densitometry. Results of (a) phospho-AKT (Ser473) and (b) phospho-AKT (Thr308) were presented after normalization with total AKT. Representative cropped blots are presented (full-length blots are presented in Supplementary Fig. S4). Results are presented as mean ± SEM of 3 independent experiments. *P < 0.05, **P < 0.01 and ***P < 0.001, significantly different from growth factor induced-hBSMCs. TCN: Triciribine.