Figure 4
Trimer-liposomes elicit stronger germinal center responses than soluble trimer immunization. (A) Representative flow plots of germinal center B cell populations (parent gate B220+ IgD-low). (B) GL7+ PNA+ IgD-low (B220+) GC B cell frequency in draining inguinal lymph nodes at 7 (red) and 21 (blue) days post-immunization. (C) Representative flow plots of total (CXCR5+ PD1+ with parent gate CD4+ CD44+) Tfh and antigen-specific (OX40+ CD25+ PDL1+) Tfh after 18 hour ex-vivo peptide stimulation. Frequencies of (D) total and (E) antigen-specific Tfh in draining inguinal lymph nodes at 7 (red) and 21 (blue) days post-immunization. Each dot in plots (B,D,E) represents one animal (n = 4–5 animals/group); *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, ordinary one-way ANOVA with Tukey post-test on each time point.
