Figure 4
From: Induction of intracellular ferritin expression in embryo-derived Ixodes scapularis cell line (ISE6)
(a) Box and whisker plot showing the distribution of ferrous iron concentration of knocked down ISE6 cells exposed to different concentrations of ferrous sulphate as determined by ferrozine assay. Cleared lysates from knocked down ISE6 cells exposed to different concentrations of ferrous sulphate, and the concentration was determined using a Micro BCA Protein Assay Kit. Ferrous iron was determined using a ferrozine assay in the absence of a reducing agent. A one-way ANOVA with Bonferonni multiple comparison tests was performed (Tables S8 and S9) (b) Box and whisker plot showing the distribution of ferric iron concentration of knocked down ISE6 cells exposed to different concentrations of ferrous sulphate as determined by ferrozine assay. Cleared lysates from knocked down ISE6 cells exposed to different concentrations of ferrous sulphate, and the concentration was determined using a Micro BCA Protein Assay Kit. Ferric iron was determined using the ferrozine assay by determining the difference between the total and ferrous iron concentrations. A one-way ANOVA with Bonferonni multiple comparison tests was performed (Tables S11 and S12). The data presented are results of three independent experiments with the ferrozine assay performed in two technical replicates for each experiment.
