Table 3 Ferrous iron concentration (pmol/ μg protein) of knockdown cells exposed to different concentrations of ferrous sulphate at 48 h.

From: Induction of intracellular ferritin expression in embryo-derived Ixodes scapularis cell line (ISE6)

Knockdown group

Ferrous sulphate concentration (mM)

0

0.1

1

2

dsEGFP

−5.02 ± 5.016

−1.39 ± 1.388

9.97 ± 1.946a

27.31 ± 0.413a

dsFER

0.00 ± 0.000

−3.87 ± 0.829

17.30 ± 0.872

50.83 ± 5.801

dsIRP

−3.41 ± 3.409

−4.22 ± 2.713

7.36 ± 0.420a

26.25 ± 1.529a

  1. Data are presented as mean ± S.D. The results for the normality and homogeneity are presented in Table S7. A one-way ANOVA with Bonferonni multiple comparison test was performed (Tables S8 and S9). Statistical significance was set as *P < 0.05. Columns with the same superscript are not significantly different at P < 0.05. The data presented are results of three separate experiments with two technical replicates for each experiment.
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