Table 7 Gene-specific primers used in this study.
From: Induction of intracellular ferritin expression in embryo-derived Ixodes scapularis cell line (ISE6)
Primers | Sequence [5′ → 3′] |
|---|---|
ISFER1 RT forward | ACTGCGAAGCTCGCATCAACAA |
ISFER1 RT reverse | ACAGGGTCTCCTTGTCGAACAT |
ISFER2 RT forward | TTGCAAGCGCTGCGAGATGC |
ISFER2 RT reverse | AGGCCCCTGCTCCCCAGAAT |
ISFER2 RT forward B | ACAAAGTGGCACGCAAAGGT |
ISFER2 RT reverse B | AAAACTCCTTCTTGTCCCCGAGCA |
ISFER1 RNAi forward | TTGCAGCAGAAAACAGCCCTTG |
ISFER1 RNAi reverse | TTGTCGAACATGTACTCTCCCAG |
ISFER1 T7 forward | TAATACGACTCACTATAGGTTGCAGCAGAAAACAGCCCTTG |
ISFER1 T7 reverse | TAATACGACTCACTATAGGTTGTCGAACATGTACTCTCCCAG |
ISIRP RT forward | GCATGCATTGAAGATGCCGT |
ISIRP RT reverse | GGAAGTAGGCCAACTCGACC |
ISIRP RNAi forward | TCGACGTACATCAAGTGCCC |
ISIRP RNAi reverse | GGAAGTAGGCCAACTCGACC |
ISIRP T7 forward | TAATACGACTCACTATAGG TCGACGTACATCAAGTGCCC |
ISIRP T7 reverse | TAATACGACTCACTATAGG GGAAGTAGGCCAACTCGACC |
16S RT forward | CTGCTCAATGATTTTTTAAATTGCTGTGG |
16S RT reverse | CCGGTCTGAACTCAGATCAAGTA |
EGFP RNAi forward | GACGTAAACGGCCACAAGTT |
EGFP RNAi reverse | TGCTCAGGTAGTGGTTGTCG |
EGFP T7 forward | TAATACGACTCACTATAGGGACGTAAACGGCCACAAGTT |
EGFP T7 reverse | TAATACGACTCACTATAGGTGCTCAGGTAGTGGTTGTCG |
