Figure 2
From: PIWI proteins contribute to apoptosis during the UPR in human airway epithelial cells
ER stress induces dynamic changes in piRNA and miRNA expression. (A) ER stress increases piRNA expression in 16HBE14o- cells. The mapped piRNA reads in NGS analysis were normalized to total mapped RNA reads and expressed as fold change over no stress control; TM (2.5 µg/ml), ALLN (100 µM). (B) ER stress reduces miRNA expression in 16HBE14o- cells. The mapped miRNA reads in NGS analysis were normalized to total mapped RNA reads and expressed as fold change over no stress control; TM (2.5 µg/ml), ALLN (100 µM). (C) Both ER stressors have a similar effect on piRNA expression. The number piRNAs significantly affected (two-fold relative to no stress control) are shown in the bar graph; ALLN (light grey), TM (dark grey), and both stressors (white). The number of piRNAs in each group is shown. (D) The ER stress distribution of piRNAs significantly increased (white) or decreased (grey) by ALLN and TM. (E) The Venn diagram of piRNAs affected by both ER stressors at 2, 6 and 9 hours. 34 piRNAs were continuously upregulated during the entire stress time course, another 34 of these RNAs were specifically induced at 6 hours only (38 specifically affected at this time point), whereas only 3 and 2 piRNAs each were induced during 2 and 9 hour time points (out of 5 specifically affected at each of this time points). (Supplemental Table 1) (F) Relative piRNA levels are significantly higher and maximal at the 6-hour time point after induction of ER stress. The piRNA levels (affected by both ER stressors) were expressed as fold change relative to no stress control, and the groups were compared with ANOVA on ranks. Significant changes (p < 0.05) are marked with an asterisk. The respective sequence length distribution profiles are provided in Supplemental Fig. 1.
