Figure 5
From: Blocking nuclear export of HSPA8 after heat shock stress severely alters cell survival
P140 peptide interacts with HSPA8 in the nucleus and hampers its functional activity. Co-localization of P140 and HSPA8 staining (red squares) as revealed by TEM in the nucleus/perinucleus (top panels) and in the cytosol (bottom left) of PBMCs collected from MRL/lpr mice that received the biotinylated P140 peptide via the intravenous route. Strings of small gold particles were also found in vesicles that might correspond to endosomes (bottom right). Immunolabeling was done using anti-biotin (for P140) and anti-HSPA8 antibodies followed by secondary antibodies. HSPA8, 15 nm-gold particles; P140, 6 nm-gold particles.
