Figure 4
From: Changes in bursal B cells in chicken during embryonic development and early life after hatching
Large B cells show enhanced expression of genes for both proliferation and differentiation when compared to those of small B cells. Single cells were produced from the bursa at E17 and small and large B cells were sorted using a cell sorter. (a–h) The mRNA levels of genes associated with proliferation and colonization of B cells in small and large B cells were evaluated using qRT-PCR as follows: (a) BAFF, (b) BAFF receptor, (c) IL-7, (d) IL-7 receptor, (e) CD40, (f) CD40 ligand, (g) CXCL12, and (h) CXCR4. (i–s) qRT-PCR was used to determine the mRNA levels of genes associated with B cell differentiation in small and large B cells as follows: (i) RAG1, (j) RAG2, (k) FOXO1, (l) FOXO3, (m) PAX5, (n) BLNK, (o) Ikaros, (p) Helios, (q) Aiolos, (r) EBF, and (s) E2A. *P < 0.05, **P < 0.01, ***P < 0.001.
