Figure 5
Effects of the NAADP antagonist 2 on Ca2+ signalling in single Jurkat T-lymphocytes. Jurkat T cells were loaded with Fura2/AM and calcium imaging and microinjection were carried out as previously described50. Arrows indicate time point of microinjection. (A), T cells were co-injected with 100 nM NAADP and increasing concentrations of 2 (n = 3–9). (B), Concentration-response curve showing the inhibitory effect of 2. (C), Co-injection of 4 µM InsP3 and 1 mM 2 and of 4 µM InsP3 or buffer alone (n = 4–7). (D), Co-injection of 100 µM cADPR and 1 mM 2 and of 100 µM cADPR or buffer alone (n = 4–9). Data are mean ± s.e.m.
