Figure 7
(A) Toxicity assay of 2 on non-proliferating myelin-basic protein (MBP) specific, CD4+ rat T cell blasts; (B and C) Protective effect of 2 in transfer experimental autoimmune encephalomyelitis (EAE); Animals (6 per group, body weight approx. 150 g) were injected i.p. twice per day with either PBS (vehicle control), nicotinic acid (50 μmol/100 g body weight), or 2 (50 μmol/100g body weight). Clinical scores indicate the degree of paralysis of tail and legs – as previously reported42. Data are presented as mean ± SD from one representative experiment (n = 6) of two independently conducted in vivo studies; (D) Effect of 2 on the localization of encephalitogenic T cells on day 4 post transfer in transfer experimental autoimmune encephalomyelitis (EAE); Animals (4 per group, body weight approx. 150 g) were injected i.p. twice per day with either PBS (vehicle control), nicotinic acid (50 μmol/100 g body weight), or 2 (50 μmol/100 g body weight). Animals were sacrificed on day 4 and the number of TMBP-GFP cells was determined for the organs displayed (n = 4 per group). Data are corrected for organ mass and presented as mean ± SEM from two independent experiments. Non parametric Kruskal-Wallis test shows statistical relevant differences (p < 0.05) in spinal cord migrating TMBP-GFP cells following treatment with 2, as compared to vehicle-treated animals.
