Table 2 Genomic DNA (gDNA) extraction yields. Amount of gDNA from one 5 µm section and the respective tissue section areas. Total numbers of samples: 131. The input of 200–1000 ng gDNA into bisulfite conversion equals input of 53–267 ng bisDNA per well in the qPCR.
gDNA yield | Samples | |
|---|---|---|
[ng] | n (out of 131) | [%] |
<200 | 6 | 4.6 |
≥200 | 125 | 95.4 |
≥400 | 110 | 84.0 |
≥1000 | 79 | 60.3 |