Table 2 Genomic DNA (gDNA) extraction yields. Amount of gDNA from one 5 µm section and the respective tissue section areas. Total numbers of samples: 131. The input of 200–1000 ng gDNA into bisulfite conversion equals input of 53–267 ng bisDNA per well in the qPCR.

From: Clinical performance of an analytically validated assay in comparison to microarray technology to assess PITX2 DNA-methylation in breast cancer

gDNA yield

Samples

[ng]

n (out of 131)

[%]

<200

6

4.6

≥200

125

95.4

≥400

110

84.0

≥1000

79

60.3