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Figure 2

From: Localising functionalised gold-nanoparticles in murine spinal cords by X-ray fluorescence imaging and background-reduction through spatial filtering for human-sized objects

Figure 2

Detection of GNP-amounts in murine spinal cords with XFI vs. ICP-MS. (A) Measurement geometry for XFI-scans with scan beam perpendicular to the spinal cord. (B) GNP mass, m (in nanogram), for the various probes differing in the functionalisation of the GNPs, i.e. with L1 peptide and PEG (labelled as “L1”) or only PEG (“PEG”), the post-injection time (from 0, 2, and 48 hours) and the degree of dilution (undiluted, 1:5, 1:20). No XFI-signals were found for the PEG-only cases. For ICP-MS, also the solutions containing the spinal cords were examined. For XFI, the maximum signal value over all scanned positions is given. (C) A typical (projected) spatial distribution of GNPs along a spinal cord (white circles indicating no gold-signal) shows a sharp localisation of the GNPs within a fraction of 1 mm. Note that ICP-MS cannot provide such spatial information. The ICP-MS error-bars are from the statistic over 3 consecutive measurements, the XFI error-bars include the uncertainty of the GNP-position along the scanning beam direction.

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