Figure 2 | Scientific Reports

Figure 2

From: Intra-amniotic Sildenafil Treatment Modulates Vascular Smooth Muscle Cell Phenotype in the Nitrofen Model of Congenital Diaphragmatic Hernia

Figure 2

Immunofluorescent Staining patterns of the left lobe of the lung at E14.5 with antibodies against CD31 (endothelial cells), ECAD (epithelial cells) and various markers of VSMC differentiation in oil, nitrofen, nitrofen plus IA dextrose and nitrofen plus IA Sildenafil groups. At E14.5, the expression of Notch3, Hes5, PDGFR-β, Desmin and α-SMA is decreased while the expression of calponin and SMMHC is increased in the nitrofen groups with no change following IA dextrose treatment. Following IA Sildenafil treatment, vSMC expression of these markers normalizes comparable to that seen in the oil control group.

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