Figure 4 | Scientific Reports

Figure 4

From: Higher primate-like direct corticomotoneuronal connections are transiently formed in a juvenile subprimate mammal

Figure 4The alternative text for this image may have been generated using AI.

Monosynaptic tracing from forearm MNs in P26 animals. (a) Experimental design for the retrograde monosynaptic tracing with EnvA-RV-ΔG-GFP from P18 to P26. Intraspinal injections of EnvA-RV-ΔG-GFP on P18 targeted to forearm MNs expressing TVA, G protein and RFP after infection by AAVs through intramuscular injections on P1. (b) Representative view of the contralateral cortex on P26 showing the absence of CM cells. Image sequences from 10 coronal sections corresponding to the M1/S1 area were superimposed. Scale bar, 1 mm (c) Representative view of the spinal cord at C5 to Th1 (longitudinal section). C, caudal, R, rostral, Lt, left, Rt, right. Arrow, midline. Scale bar, 200 μm. (d) Example of a brain stem coronal section. The positions of GFP positive neurons are marked (upper). Red, a fluorescent Nissl staining by Neurotrace Red. Scale bar, 1 mm. (e,f) Higher power view of the selected regions in (d). Scale bar 100 μm. The images are representative of five independent experiments.

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