Figure 4
Increase in CD8 T cell effector phenotype and function following challenge is unique to Ad-SF40L immunization. (A) Schematic diagram of the animal study with BALB/c mice. Mice were necropsied for tissue collection before or after challenge. (B) Flow cytometry was used to determine the TEM population (CD3+ CD8a+ CD44+ CD62L− CCR7−) in the spleen. Induction of the TEM population as a result of the RSV challenge is shown as a ratio (TEM after challenge/TEM before challenge). A ratio of 1 indicates no change in the TEM population after challenge. (C) Intracellular cytokine staining was done following 4-hour ex-vivo stimulation with F85–93 peptide to analyze the number of CD8α+ TNF-α+ cells in the spleen using flow cytometry. Fold change of this population as a result of the challenge is shown (after challenge/before challenge). Data shown is mean ± SEM representative of 2 independent experiments; n = 4 per group; *p < 0.05, **p < 0.01 (one-way ANOVA with Bonferroni posttest).
