Figure 6
CD8 T cell effector function is altered following challenge of FIRSV-immunized mice unlike in Ad-SF40L immunization. (A) Schematic diagram of the animal study timeline. Adoptive CD8 T cell transfer was done from immunized donors either before (BC) or after (AC) challenge into naïve recipient mice. CD8 T cells were isolated from spleens of donor mice using a magnetic bead kit and checked for purity prior to transfer. (B) Four days post-challenge, lungs from the recipient mice from BC transfer (left) and AC transfer (right) were collected for viral titer determination using plaque assay. Ad-SF40L was compared to all groups (left) and FIRSV was compared to all groups (right). Comparisons between other groups showed no statistically significant differences. Secreted levels of IFN-γ (C) and TNF-α (D) were determined, using the Luminex system, in spleens of recipient mice from BC transfer (left) and AC transfer (right) following 48-hour ex-vivo stimulation with F85–93 peptide. Ad-SF40L was statistically different from all groups for both cytokines. Data shown is mean ± SEM representative of 2 independent experiments; n = 4 per group; *p < 0.05, **p < 0.01, ***p < 0.001 (one-way ANOVA with Bonferroni posttest).
