Figure 1
From: Cancellation of Bessel beam side lobes for high-contrast light sheet microscopy
(a) Generation of the droplet illumination in a light sheet imaging system. The amplitude of an expanded laser beam was modulated by an SLM and focused by an objective lens (Oill) of NA = 0.25. Fluorescence light from a test sample was collected by a second orthogonal objective lens (Ocol) of NA = 0.35 and a tube lens L3. A CCD camera was used to acquire the image by integrating the fluorescence signal emitted during a \(\hat{y}\) scan. Li lenses; λ/2 half-wave plate; P linear polarizer; F bandpass filter. (b,c) To generate the droplet illumination, a set of amplitude binary masks composed of two concentric rings of inner and outer radii, r2 and r1 respectively, and thickness dr were displayed by the SLM.
