Figure 4 | Scientific Reports

Figure 4

From: Correlative Light-Electron Microscopy detects lipopolysaccharide and its association with fibrin fibres in Parkinson’s Disease, Alzheimer’s Disease and Type 2 Diabetes Mellitus

Figure 4The alternative text for this image may have been generated using AI.

Representative confocal micrographs of healthy platelet poor plasma (PPP) with added LPS to show optimization of detection of LPS, by using anti-E.coli LPS antibody and a secondary antibody (1:200 dilution for primary and secondary antibodies). We used five different LPS concentrations, and estimated that 0.5 μgL−1 LPS is the lowest detectable concentration. For clarity, we inverted the micrographs, followed by applying the “find edges” function in ImageJ (FIJI), to show the decreasing fluorescence LPS signal with decreasing concentrations added.

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