Figure 3
From: NTG-101: A Novel Molecular Therapy that Halts the Progression of Degenerative Disc Disease
Effect of intra-discal injection of NTG-101 in rodent needle puncture disc injury model. (a) Representative images showing Safranin-O stained rodent IVD tissue sections (uninjured, healthy age-matched control), injured IVDs injected with either PBS (1X, pH = 7.2), excipient solution or NTG-101 (Scale bar: 500 µm). Panel (b) shows the histogram analysis showing mean score ± S.D. of the morphology (M), cellularity (C), Safranin O staining intensity (I) and total health score (M + C + I). ***p-value for NTG-101 injected group of animals is with respect to the group of injured IVDs injected with PBS only. Panel (c) represents images of immunohistochemistry for Aggrecan, Col2A1, Brachyury, Oct4, IL-1β, MMP-13, Cox-2 and PGE2 in age-matched, uninjured, healthy rat tail IVDs as well as injured IVDs injected with PBS (1X) or NTG-101 (Scale bar: 25 µm). Panel (d) represent histograms showing average values of IHC total score obtained for aggrecan, Col2A1, Brachyury, Oct4, IL1-β, MMP-13, Cox-2 and PGE2. Each histogram shows mean ± SD, *p ≤ 0.05.
