Figure 6
From: Inactivation of NUPR1 promotes cell death by coupling ER-stress responses with necrosis
NUPR1 deficiency induces a decrease in the expression of ER stress genes. Total RNA was extracted from MiaPaCa2 cells transfected with siCtrl, siNUPR1-1 or siNUPR1-2 for 72 h to evaluate the mRNA levels of the genes involved in ER stress response using RT-qPCR (A). Western blot analysis was performed to evaluated protein levels of expression of IRE1α, ATF6, PERK and BIP in NUPR1-depleted cells. Data are means of triplicates ± SEM. Statistically significant differences from siCtrl (*p < 0.05, **p < 0.01, ***p < 0.001) was shown.
