Figure 2

Modified Verhoeff-Van Gieson staining of the elastic laminae in the tunica media. Cryosections of the suprarenal aortic segment of Vwf+/+ and Vwf−/− mice, continuously infused with AngII for 28 days, were stained for elastin using a Modified Verhoeff-Van Gieson staining. Representative sections of every aneurysm stage per group are depicted and the framed areas are shown in greater detail below the original picture. The elastic laminae are visualized by the dark purple lines in the media of the aortas and elastin breaks are indicated by an arrowhead. Collagen appears red/purple on the modified Verhoeff-Van Gieson staining. Images were acquired with a Hamamatsu NanoZoomer-SQ digital slide scanner.