Figure 4

VWF content in the suprarenal aortic tissue of Vwf+/+ mice after AngII-induced AAA formation. Histological examination of the VWF content in cryosections of the suprarenal aortic segments of Vwf+/+ mice, which received AngII for 28 days. VWF was stained using rabbit anti-VWF antibodies (purple color). Cryosections stained without primary antibody were used as negative controls. Nuclei were visualized using a methyl green counter stain (green color). Representative sections of every aneurysm stage per group are depicted and the framed areas are shown in greater detail below the original picture. Pictures were taken using a Hamamatsu NanoZoomer-SQ digital slide scanner.