Figure 4

Comparison of qRT-PCR and RNAseq data for selected Clostridium difficile strain 630 genes. For each individual gene, expressional changes determined by RNAseq (up-hatched columns) and by qRT-PCR (down-hatched columns) are shown relative to the BHIS control, and show good correlation between the two datasets (R2 = 0.97). rpsJ, gyrA and adk were used as reference genes.