Table 3 PCR primers.
Gene | Locus | Description | Primer | Sequence (5′ → 3′) | Binding position | Product size (bp) | Annealing temperature (°C) | Reference |
|---|---|---|---|---|---|---|---|---|
rho | CD3487 | Transcription termination factor Rho | rho-F rho-R | CATCAAGCAATAAATCATCTC CTGGTTCTAGGATGGATGATG | 141–293 | 153 | 57 | Metcalf et al.96 |
gyrA | CD0006 | Gyrase subunit A | gyrA-F gyrA-R | CTCGTATTGTTGGGGACGTT ATCCCCATCAACAGAACCAA | 197–342 | 146 | 57 | Denève et al.97 |
adk | CD0091 | Adenylate kinase | adk-F adk-R | GTGTATGTGATGTATGCCAAG CCTAAGGCTGCGACAATATC | 443–638 | 196 | 57 | Metcalf et al.96 |
rpsJ | CD0072 | 30 S ribosomal protein S10 | RpsJ-F rpsJ-R | GATCACAAGTTTCAGGACCTG GTCTTAGGTGTTGGATTAGC | 101–251 | 151 | 57 | Metcalf et al.96 |
groES | CD0193 | 10 kDa chaperone | groES-F groES-R | AGTTTTACCAGGAGCAGCTAAAG CCTTATCTCCCACTGTCAATTCC | 75–190 | 116 | 57 | This study |
groEL | CD0194 | 60 kDa chaperone | groEL-F groEL-R | TTGCTGGAGGAGTAGCTGTTG AAAAGCAGTTCCTCCACCAG | 1109–1251 | 143 | 57 | This study |
fliC | CD0239 | Flagellin subunit | fliC-F fliC-R | TGATGATGCTGCTGGACTTG ACGAACCTTCTGCTGTTTGTAC | 120–238 | 119 | 57 | This study |
fliD | CD0237 | Flagellar cap protein | fliD-F fliD-R | AGCTGGACAAATTGCCAGTG CCTTGGTCATCAGTTACATCAGC | 621–734 | 114 | 57 | This study |
tcdA | CD0663 | Toxin A | tcdA-F tcdA-R | AGCTTTCGCTTTAGGCAGTG ATGGCTGGGTTAAGGTGTTG | 1121–1250 | 129 | 57 | This study |
spoVB | CD3498 | Stage 5 sporulation protein B | spoVB-F spoVB-R | ATTCAGGGAATGGGAAAACC TTAATCATGGCTGCCACAAA | 1165–1325 | 161 | 57 | This study |
