Figure 9
1H-NMR analyses of FTGMs without Galf sugar chains (FTGM-HCls) from strains A1151 (WT), ∆cmsA, ∆cmsB, ∆cmsAB, ∆cmsA::cmsA, and ∆cmsB::cmsB. Total GM (FTGM + OMGM) was extracted and further purified FTGMs were prepared by treatment of GM with 0.5 M NaBH4/0.1 M NaOH for 24 h (β-elimination), FTGM-HCl was prepared by acid hydrolysis of the purified FTGMs (0.1 M HCl for 60 min). The signals A (at 5.104 ppm), B (5.233), C (5.216), and D (5.054) of the 1H-NMR spectra are derived from H-1 at the C-1 position of the underlined Man residues in the structures -(1 → 6)-α-Man-(1 → 2)-α-Man-(1 → 2)-α-Man-(1 → 2)-α-Man-(1 → 6)- (A), -(1 → 6)-α-Man-(1 → 2)-α-Man-(1 → 2)-α-Man-(1 → 2)-α-Man-(1 → 6)- (B), -(1 → 6)-α-Man-(1 → 2)-α-Man-(1 → 2)-α-Man-(1 → 2)-α-Man-(1 → 6)- (C) and -(1 → 6)-α-Man-(1 → 2)-α-Man-(1 → 2)-α-Man-(1 → 2)-α-Man-(1 → 6)- (D). The signals A′ and D′ at 5.1 and 5.05 ppm of the 1H-NMR spectra are from H-1 at the C-1 position of the underlined Man residue in t-Man-(1 → 6)-α-Man- and t-Man-(1 → 2)-α-Man-. Asterisks indicate an unidentified NMR signal. The proton chemical shifts were referenced relative to internal acetone at δ 2.225 ppm.
