Table 3 Differential expression of selected P. aeruginosa genes.

From: Pseudomonas aeruginosa mutants defective in glucose uptake have pleiotropic phenotype and altered virulence in non-mammal infection models

Locusa

Name

Fold Change

Description

PAO1 + vs. PAO1−

GUN + vs. PAO1+

GUN + vs. PAO1−

Genes up-regulated in GUN mutant b

PA0105

coxB

0.8

8.2

6.5

cytochrome C oxidase subunit

PA1174

napA

0.8

4.8

3.6

nitrate reductase subunit

PA1430

lasR

1.0

1.7

1.8

transcriptional regulator LasR

PA2426

pvdS

2.5

1.9

4.8

sigma factor PvdS

PA2570

lecA

0.7

4.7

3.1

LecA lectin

PA3361

lecB

0.6

5.2

2.9

fucose-binding lectin PA-IIL

PA3477

rhlR

0.8

2.4

1.9

transcriptional regulator RhlR

PA3478

rhlB

0.8

3.0

2.3

rhamnosyltransferase subunit B

PA3479

rhlA

0.7

3.3

2.5

rhamnosyltransferase subunit A

PA3622

rpoS

0.9

2.6

2.4

sigma factor σS

PA3724

lasB

0.5

7.8

3.5

elastase LasB

PA4210

phzA1

0.4

6.2

2.7

phenazine biosynthesis protein

PA4211

phzB1

0.5

7.2

3.2

phenazine biosynthesis protein

PA5348

PA5348

0.8

3.0

2.4

probable DNA-binding protein

Genes down-regulated in the GUN mutant b

PA0576

rpoD

1.1

0.4

0.5

sigma factor σD

PA0893

argR

0.6

0.6

0.4

transcriptional regulator ArgR

PA0895

aruC

0.5

0.3

0.2

acetylornithine aminotransferase

PA2259

ptxS

2.9

0.5

1.4

transcriptional regulator PtxS

PA2264

PA2264

3.4

0.5

1.5

conserved hypothetical proteinc

PA2320

gntR

3.7

0.3

1.1

GntR transcriptional regulator

PA4006

nadD

0.9

0.5

0.5

NAM adenylyltransferase

PA4920

nadE

1.5

0.5

0.8

NAD synthetase

PA5100

hutU

0.8

0.0

0.0

urocanate hydratase

PA5105

hutC

0.8

0.2

0.1

Transcriptional regulator HutC

Genes for glucose IM transporters deleted in this work

PA2262

kguT

1.3

0.3

0.4

2-ketogluconate transporter

PA2322

gntP

35.5

0.0

0.6

gluconate permease

PA3187

gltK

52.0

0.1

6.7

ABC transporter ATP-binding protein

PA3188

gltG

31.6

0.0

0.4

sugar ABC transporter permease

PA3189

gltF

3.5

0.3

0.9

probable permease of ABC sugar transporter

  1. aBoldface characters, genes analysed by RT-qPCR.
  2. bGenes up- and down-regulated in GUN mutant with respect to PAO1 (with and/or without glucose).
  3. cThe protein contains a domain belonging to the Gluconate 2-dehydrogenase subunit 3 family (InterPro IPR027056).
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