Figure 1 | Scientific Reports

Figure 1

From: ROCK2, but not ROCK1 interacts with phosphorylated STAT3 and co-occupies TH17/TFH gene promoters in TH17-activated human T cells

Figure 1

ROCK2 but not ROCK1 interacts with pSTAT3 in both cytoplasmic and nuclear fractions of human T cells stimulated by TH17 skewing conditions. Human peripheral blood CD4+ T cells were treated with indicated doses of KD025 and then stimulated under TH17-skewing conditions for 2 hours. (a) Total cell extracts were prepared and analyzed by Western blot for pSTAT3 and pMLC expression. (b) Co-immunoprecipitation of pSTAT3 by anti-ROCK2 and anti-ROCK1 antibody in the nuclear fraction (c) Co-immunoprecipitation of pSTAT3 by anti-JAk2 antibody from total cell lysates of human T cells. (d) Co-immunoprecipitation of ROCK2 and ROCK1 with an anti-pSTAT3 antibody in both cytoplasmic and nuclear fractions. Co-immunoprecipitation of p300 (e) or JAK3 (f) by anti-ROCK2 and anti-ROCK1 antibody in the nuclear (e) or cytoplasmic (f) fractions. Numbers indicate ratio between the precipitated protein and the input (e). One representative of three different experiments is shown. Full-length gels are included in Supplementary Fig. 1.

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