Figure 5
From: A Rabbit Corneal Endothelial Dysfunction Model Using Endothelial-Mesenchymal Transformed Cells
Histological examination by Hematoxylin and Eosin (HE), smooth muscle α-actin (α-SMA) and vimentin staining. Histological analysis of EMT-RCECs group, Scrape-group, Fresh RCECs group and untreated normal corneas (top panel: Hematoxylin and Eosin (HE) staining; middle panel: smooth muscle α-actin (α-SMA) staining, bottom panel: vimentin staining at 6 weeks after injection). Injected EMT-RCECs were engrafted on Descemet’s membrane with fibroblast-like morphology. α-SMA staining was also observed in the stroma of the Scrape-group, while Fresh RCECs group and normal RCECs only showed weak staining. Vimentin staining was similar in all groups. Scale bars = The upper left panel 500 μm, center panel 50 μm. *Descemet’s membrane was intact. Transplanted EMT-RCECs formed a confluent layer on the Descemet’s membrane. (arrow). Corneal endothelial cells formed monolayers (arrow head). Abbreviation S: Corneal stroma, *Descemet’s membrane.
