Figure 1
DPG3 activated DC-SIGN, AKT, regulated FOXO1 phosphorylation/expression was required for the survival effect of DPG3 in MDDSCs. Immature monocytes isolated from human peripheral blood mononuclear cells (PBMCs) were infected with Pg381, DPG3 and MFI for 6 hours at 1 MOI. (A) Immunoblot analysis of DC-SIGN, p/tAKT, p/tFOXO1 and BIM in MDDSCs and MoDCs with GAPDH as loading control. Immunoblot detected increased expression of DC-SIGN, p/tAKT, p/tFOXO1 and decreased expression of BIM in DPG3- infected MDDSCs compared with uninfected control MoDCs. (B) DC-SIGN was blocked with HIV-gp120 (6ug/ml) for 30 minutes before infection. Notably, DC-SIGN expression and internalization was abolished by its antagonist HIV-gp120 and consequently the downstream signaling cascade also blocked as evident by dephosphorylated or decreased expression of pAKT (B,D), pFOXO1 (B,E) and increased expression of BIM (B,F). (C–F) Quantification analysis of DC-SIGN (C), and relative ratio of p/tAKT (D), p/tFOXO1 (E) and BIM (F) after normalized with the internal control GAPDH. Results are from one representative of three independent experiments. Data were analyzed by performing one-way ANOVA with Tukey’s post hoc using Prism GraphPad. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001.
