Figure 5
From: Luminescence lifetime encoding in time-domain flow cytometry
Impact of signal and background intensity. (a) Simulated dependence of the lifetime on the number of collected photons. Integration time range θ = 40 ns, no background signal, 10 ps bin width. Each data point represents the mean or standard deviation of 1000 repetitions (corresponding to 1000 events in FCM). The mean calculated lifetime turns out to be independent of the photon count number. The standard deviation and therefore the precision benefits from more counts. (b) Simulated dependence of the lifetime on the signal-to-background ratio. Integration time range θ = 100 ns, 100 photons per event, 1000 repetitions (i.e. events in FCM). The dotted green line represents the analytical expression given by Eq. (8).
