Figure 6
From: Luminescence lifetime encoding in time-domain flow cytometry
Influence of technical parameters. (a) Lifetime distribution of code B obtained from LT-FCM with varying neutral density filter transmittance T in the detection channel. Fits of Gaussian distributions have been added for determination of mean values and standard deviations and as guides to the eye. For each transmittance value, ≈8000–12000 beads were measured and the time range was set to θ = 20 ns. There is a distinct shift of the distributions towards longer mean lifetimes with increasing filter transmittance. (b) Dependence of the obtained fluorescence lifetime on the applied pressure for code D. Integration time range θ = 50 ns, around 1000 beads/measurement, photon count numbers approx. between 50 and 160 per bead. An increased pressure, and therefore a higher flow speed and a shorter interaction time, results in slightly longer measured lifetimes. However, the standard deviation of the measured distributions is much larger than the variation due to the flow speed.
