Figure 7
From: Luminescence lifetime encoding in time-domain flow cytometry
Lifetime distributions from LT-FCM. (a) Lifetime distributions of five lifetime codes. The curves with the solid lines were obtained from measurements of pure samples and scaled to the respective peaks in mixture for comparison. Around 1000–4000 beads per code were measured. Photon count numbers range from around 50 to almost 700, integration time range θ = 20 ns and an ND filter combination with T = 6.3% was used. (b) Comparison of experimental and numerical lifetime distributions exemplarily for two codes. Exploiting the lifetimes from ensemble measurements, cf. Table 1, it is possible to numerically generate lifetime distributions that are in good agreement with measured distributions which underlines the applicability of our simulation method.
