Table 1 Lifetime codes and respective luminophores with measurement parameters, mean bead diameter D and obtained ensemble (reference) lifetimes calculated as intensity-weighted mean lifetimes from multi-exponential decay fits.

From: Luminescence lifetime encoding in time-domain flow cytometry

LT code

Luminophore

D/μm

λex/nm

Em. range/nm

λdet/nm

τ/ns

A

Red5 400%

6.5

488

602–800

645 LP

1.72

B

Red/Red5 100:300%

6.3

488

545–800

645 LP

2.71

C

Red 50% (400%*)

9 (6.9*)

488

559–718

590 LP

5.54

D

Pink 400%

6.8

488

528–708

645 LP

7.91

E

CdSe/CdS/ZnS QDs

6.2 ± 0.6

488

621–680

645 LP

22.6

  1. The luminophore designation are trade names of PolyAn GmbH and the percentages denote the loading concentration (*two dye loading concentrations used). Code B is a mixture of codes A and C. Further details on the spectroscopic properties can be found in the Supplementary Information.
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