Figure 5

Open-chest LAD ischemia/reperfusion with 50 min ischemia and 4 weeks reperfusion in IL-23R and IL-23R-Y416FΔICD signaling deficient mice. (A) Schematic illustration of the open-chest LAD experiment with IL-23R and IL-23R-Y416FΔICD mice. Baseline measurement were performed at day -1, LAD ligature and 50 min of ischemia was done at day 0 followed by 4 weeks of reperfusion and analysis at day 1, 4, 7, 14, 21 and 28. (B) MRI analysis of ejection fraction, endsystolic- and enddiastolic volume before (baseline) and after 24 h, 4 d, 7 d, 14 d, 21 d and 28 d of reperfusion. Results are mean ± S.E.M. of 7–8 animals/group (IL-23R n = 7; IL-23R-Y416FΔICD n = 8; baseline: IL-23R n = 3; IL-23R-Y416FΔICD n = 4). (C) Immunostaining and quantitative analysis of αSMA protein in the left ventricle (LV) after 28 days of reperfusion; 40x magnification; scale bars, 50 µm. Results are mean ± S.E.M. of 7–8 animals/group (IL-23R n = 7; IL-23R-Y416FΔICD n = 8). (D) Immunostaining with HAbP and quantitative analysis of HA amounts in the LV after 3 weeks of reperfusion. Representative images of the LV after 3 weeks of reperfusion and quantitative image analysis; 40x magnification; scale bars, 50 µm Results are mean ± S.E.M. of 7–8 animals/group (IL-23R n = 7; IL-23R-Y416FΔICD n = 8). (E) Representative Gomori’s 1-step trichrome staining of heart sections and quantitative analysis of infarct size/LV after 28 days of reperfusion; scale bars, 1 mm. Results are mean ± S.E.M. of 5–6 animals/group (IL-23R n = 6; IL-23R-Y416FΔICD n = 5). (F) Calculated amounts of ¹⁹F as indicator of macrophages infiltration after 24 h of reperfusion by magnetic resonance imaging as described in “Experimental procedures”. (G) Ischemic areas of hearts after 24 h of reperfusion was visualized and calculated by magnetic resonance imaging as described in “Experimental procedures”.