Figure 3
Colon sections of 10 week-old (A) and 19 month-old mice (B) stained with Alcian blue to reveal goblet cells and antibody to cleaved caspase 3 to reveal apoptotic cells. (B and detail in panel (C) A proportion of goblet cells in the in colonic crypts of 19-month-old mice but not in 10-week-old mice stained positive for cleaved caspase 3 (arrowed). Ileum sections of 10 week-old mice (D) and 19 month-old mice (E) stained with Alcian blue to reveal goblet cells. Significantly greater number of goblet cells per villus were observed in 10 week-old mice than 19 month-old mice (H). Immunostaining for Ki67 revealed more strongly positive proliferative cells in colon sections of the 10 week-old mice (black arrows) than 19 month-old mice (F,G).
