Figure 4 | Scientific Reports

Figure 4

From: Age-associated Impairment of the Mucus Barrier Function is Associated with Profound Changes in Microbiota and Immunity

Figure 4

Representative pictures of PAS/Alcian Blue staining of colon (A) and ileum (E) of 2 month-old mice, and of colon (B) and ileum (F) of 19 month-old mice. Scale bars: 100 μm. Muc2 staining (in green; cell nuclei in blue) of colon of young mice (C) and old mice (D). Scale bars: 50 μm. Mucus thickness measured (10 measurements per section, 2 sections per animal) in 5 colonic tissues of young and old mice, respectively. Pooled mucus measurements are presented in panel G. ***Indicates statistical difference at P < 0.001. Representative pictures of PAS/Alcian Blue staining of colonic SILT (H).

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