Figure 5
From: Quenched Stochastic Optical Reconstruction Microscopy (qSTORM) with Graphene Oxide
(a) STORM reconstruction of I3K peptide fibrils at the solid/liquid surface. On the left of the image a graphene oxide flake is present. The graphene oxide effectively reduces the background signal due to surface adsorbed free fluorophores and fluorescently labelled peptide monomers. (b,c) Show the contours of peptide fibres located using image analysis software (black lines, FiberApp)47. The surrounding localisations of two example fibrils can be compared where graphene oxide is present (b) and where it is not (c). The presence of graphene oxide allows for potentially more accurate analysis using snake based algorithms and reduces ambiguity when determining localisation distributions along a fibril. The sample preparation and image analysis was identical to that presented in a previous work with the addition of a GO coated substrate28.
