Figure 1
From: A standardized herbal extract PM014 ameliorates pulmonary fibrosis by suppressing the TGF-β1 pathway
PM014 ameliorates pulmonary fibrosis induced by BLM in mice. (a–f) Mice were administered intratracheally once with BLM (5 mg/kg) at day 0 and subsequently received PM014 (50, 100, or 200 mg/kg) daily from day 2 to day 13 after BLM exposure. (a) The experimental schedule was represented. (b) Representative images of pulmonary tissues stained with hematoxylin and eosin (H&E) or Masson’s trichrome staining were shown from control, BLM, and BLM + PM014 (200 mg/kg) group. (c,d) Ashcroft scoring and morphometric analysis of collagen were performed to determine the extent of lung fibrosis. (f) Hydroxyproline content used to evaluate collagen content was quantified in the lung tissues. (g–i) Mice were administered intratracheally once with BLM (2 mg/kg) at day 0 and subsequently received PM014 (200 mg/kg) daily from day 2 to day 21 after BLM treatment. (g) The experimental schedule was represented. (h) Representative images of lung tissues stained with Masson’s trichrome staining are shown from control, BLM, and BLM + PM014 (200 mg/kg) group. (i) Ashcroft scoring system was adopted to determine the extent of lung fibrosis. Data are presented as mean ± SE, n = 4–8 for each experimental group, *P < 0.05; **P < 0.01; ***P < 0.001.
