Figure 3
From: A standardized herbal extract PM014 ameliorates pulmonary fibrosis by suppressing the TGF-β1 pathway
PM014 inhibits pulmonary inflammation in lung fibrosis induced by BLM. Mice were injected with BLM (5 mg/kg) on day 0, followed by PM014 (50, 100, or 200 mg/kg) daily for consecutive 12 days, beginning on day 2 after BLM administration. (a–c) Bronchoalveolar lavage fluid (BALF) analysis was carried out by counting the number of total inflammatory cells, macrophages, and lymphocytes. (d–i) The level of pro-inflammatory cytokines from lung tissues of mice were analyzed by ELISA. The investigated cytokines were TNF-α, IL-1β, IL-6, TGF-β1, IL-17A, and IL-23. NS indicates no significant differences were found among groups. Data are presented as mean ± SE, n = 4–11, *P < 0.05; **P < 0.01; ***P < 0.001.
