Figure 4
Increased epithelial features in response to RGS2 downregulation. (a) Quantitative comparison of migrating properties of the sh-clones, presented as percentage of area of gap closure compared to the starting point. (n = 4; 24 h p = 0.042, 48 h p = 0.017, by two-tailed Student’s t test). (b) Representative images of scratch assay at analysed time points. (c) Magnification of the scratch for visualization of migration pattern. (d) Phase imaging of the Sh-clones for demonstration of morphology and growth pattern. (e) Quantification of clone formation (n = 4, p = 0.0051, by two-tailed Student’s t test). (f) Images of colony formation ability of low seeded clones stained with crystal violet.
