Figure 6
Affected Pathways and EMT initiation in response to RGS2 depletion. (a) Evaluation of STAT3 and AKT proteins in response to RGS2 knockdown using WB. (b) Overview of evaluated EMT markers from a PC gene expression panel (this data represent a single replicate). (c) qRT-PCR quantification of mRNA expression of EMT associated TWIST1 and E-cadherin of TWIST1 and CDH1 (n = 3; TWIST1 p = 0.021, CDH1 p = 0.034, student’s t test). (d) Representative western blots for corresponding protein expression. The blots were cropped and gels were run at the same conditions. The original pictures for the evaluation of the clones can be found in Supplementary Fig. S4). (e) Immunocytochemistry (ICC) analysis of localization of E-cadherin expression (red) and nuclei counterstained with DAPI (blue).
