Figure 9
Proliferation of endothelial cells producing a 79 amino acid vasoinhibin. (a) Representative fields of bovine umbilical vein endothelial cells (BUVEC) transduced or not (Ctl) for 24, 48, or 72 h with lentiviral vectors coding for the 79- and 123-residue vasoinhibins (Vi) or with the empty vector (Neg). Scale bar, 500 μm. (b) Proliferation is further illustrated by the number of small and brilliant Hoechst-stained nuclei (arrowheads) characteristic of dividing cells at 72 h post-lentiviral transduction. Scale bar, 250 μm. (c) BUVEC density following 24, 48 and 72 h of lentiviral transduction. Values are means ± SEM of 3 independent experiments (*p < 0.001 vs. Ctl and Neg). (d) Western blot analysis of BUVEC conditioned media at 0, 24, 48, and 72 h after transduction with lentiviral vectors coding for Vi79 (~9 kDa) and Vi123 (~14 kDa) or the empty vector (Neg).
