Figure 6
From: Amplification of Mitochondrial Activity in the Healing Response Following Rotator Cuff Tendon Injury
(A) Determination of mitochondrial superoxide using MitoSox showing increased superoxide in hypoxic tenocytes with normoxic control. Images in the left column show nuclear staining with DAPI; the images in the middle column show expression of superoxide while the images in the right column show overlay of MitoSox with DAPI. Images were acquired at 20x magnification using CCD camera attached to the Olympus microscope. (B) The image shows quantification of the mitochondrial superoxide. The intensity of protein expression as observed through immunofluorescence was acquired and the mean fluorescence intensity (MFI) was normalized to 100 cells. The graphs represent MFI mean values with standard error. The statistical significance of each hypoxic groups vs normoxic groups are represented in the figure (n = 4; NS – non-significant, *P < 0.05, **P < 0.01 and ***P < 0.001).
