Figure 7
Determination of calcification in human vascular tissue (calcified and control). (A) Volume rendered image; (B) CT image; (C) PET image, with NaF tracer, of vascular tissue. Yellow, purple and orange boxes indicate the areas selected for tissue sectioning where Brightfield and Fluorescence images of calcification were taken. (D–G) Brightfield and Fluorescence (Alizarin S, Fluorescein-BP, DAPI) images of tissue in the yellow box which show high CT activity. (H–K) Brightfield and Fluorescence (Alizarin S, Fluorescein-BP, DAPI) images of tissue in the purple box which show high PET activity. Sections were incubated with Fluorescein-BP (1 μM, 2 hours), Alizarin S (2%, 10 minutes) and DAPI (500 nM, 5 minutes) and subsequently imaged. Black boxes indicate region of Brightfield image where Fluorescence images were taken. Control sample, PET image (L) and Fluorescence (Alizarin S, Fluorescein-BP and DAPI, M). Scale bars = 2.0 mm for Brightfield and 100 µm for Fluorescence.
