Figure 1 | Scientific Reports

Figure 1

From: Computational affinity maturation of camelid single-domain intrabodies against the nonamyloid component of alpha-synuclein

Figure 1

Identifying recombinant intrabodies using FLI-TRAP. (a) Schematic of engineered genetic selection for co-translocation of cognate intrabody-antigen pairs via the Tat translocase, TatABC. Here, an immune library of NAC-focused VHH genes was fused to a Tat signal peptide, namely spTorA, and co-expressed with a chimera between the antigen α-syn(A53T) and the reporter enzyme TEM1-Bla. By demanding cell growth on selective amounts of β-lactam antibiotics (e.g., Carb), intracellularly stable VHH clones that bind α-syn(A53T) can be readily isolated from the library. (b) Iterative workflow based on estimates from experiments for updating computational models and making new predictions.

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