Figure 1
From: Diffracted X-ray Blinking Tracks Single Protein Motions
Diffracted X-ray blinking method. (a) Schematic representation of the classical DXT (upper) and diffracted X-ray blinking (DXB) methods (bottom). (b) DXB with a monochromatic X-ray from a synchrotron-radiation facility for monitoring the temperature-dependent motion of gold nanocrystals immobilised on a polyimide-substrate surface. The X-ray-diffraction intensities of the pixels at the Au(111) positions (inset of the figure) were treated with auto-correlation functions, and the decay constant was discussed in the context of DXB. (c) DXB with a laboratory X-ray source (Lab-DXB). Gold nanocrystals were labelled on AChBP molecules, which were immobilised on the polyimide-substrate surface. The motions of the AChBP molecules were investigated in the presence and absence of ACh in the experimental solution.
