Figure 2 | Scientific Reports

Figure 2

From: A single-cycle replicable Rift Valley fever phlebovirus vaccine carrying a mutated NSs confers full protection from lethal challenge in mice

Figure 2

Effects of scMP-12-mutNSs replication on PKR abundance and host transcription. (a) Levels of total PKR in scMP-12-mutNSs-infected cells. HeLa cells were infected with indicated viruses at a m.o.i. of 1 and harvested at 8 h p.i. Cell lysates were subjected to Western blot analysis using anti-PKR antibody (top panel), anti-MP-12 antibody (middle panel), and anti-beta-actin antibody (bottom panel). (b) General RNA transcription in scMP-12-mutNSs-infected cells. Vero E6 cells were treated with 5EU for 1 h at 16 h after infection with indicated viruses at a moi of 1 and fixed with 4% paraformaldehyde. The cells were treated with Alexa Fluor 488 conjugated azide to visualize 5EU-labeled RNA and further stained by anti-N antibody followed by Alexa Fluor 594 conjugated secondary antibody (N protein).

Back to article page