Figure 1

Analysis of the yidC mRNA transcripts in M. tuberculosis. Transcript of yidC, prepared from wild-type M. tuberculosis, was subjected to reverse transcription for the cDNA synthesis followed by PCR amplification of the junction sequences across various ORFs in the locus using designated primer pairs, as indicated (A). Presence of amplicon of ~1.1 kb in the reaction PCR-1 in the presence of reverse transcriptase (RT, +) suggests expression of yidC as an operon with upstream genes, whereas no amplification was observed in the reaction PCR-2 indicating that the downstream gene MRA_3959 is transcribed from an independent promoter situated upstream to its ORF; independent expression of MRA_3959 is also verified by PCR-3 indicating the amplification of expected size of ~700 bp (B). Reactions performed without RT (-) in (B) were used as negative control to verify specificity of RT-PCR, whereas amplification with genomic DNA (gDNA) was used as positive control for each primer pair.